ABSTRACT
The objective of this study was to observe the morphometric changes in the testes of albino rats exposed to lead acetate. Experimental animal study. This study was conducted from January to April, 2009 at National Institute of Health Islamabad. Animals were obtained from the animal house of N.I.H and were divided into two groups A and B. The animals in group A were used as control, while those of groups B were treated with lead acetate that was given intraperitonially in the dose of 4mg/kg body weight, 5 days a week for 6 weeks. After 6 weeks the animals of group A [control] and group B [experimental] were sacrificed by an overdose of ether anesthesia. The testes were fixed in formalin and then processed for paraffin embedding. Five micrometer thick section were cut, stained with hematoxylin and eosin, and observed microscopically for germinal epithelium thickness and the basement membrane of seminiferous tubules. The histological comparison of testes of both groups of animals showed that after six weeks, the width of germinal epithelium and the number of spermatogenic cells had decreased in the test group as compared to the control group [p<0.05] and in majority of the seminiferous tubules, the basement membrane was disrupted
ABSTRACT
To investigate the variations in the total number of segmental arteries in human kidneys obtained from cadavers of the local adult population. Observational Study. This study was conducted at Anatomy Department of University of Health Sciences, Lahore, for a period of one year from October 2006 to October 2007. Forty four adult human kidneys were obtained after autopsy; they were randomly divided in two groups A and B of right and left kidneys respectively. Simple blunt dissection and corrosion cast techniques were used to study the number of segmental arteries. Statistical analysis was carried out by using SPSS version 16.0 and STATA version 8.0. Segmental arteries were present in 100% of specimens of groups A and B; variations were seen in the number of segmental arteries of both groups. The segmental branches of renal artery in local population showed variations different from those reported in the earlier work carried out in other countries
Subject(s)
Humans , Renal Artery , CadaverABSTRACT
The objective of this study was to observe changes in spermatogenesis testes of albino rats exposed to intraperitoneal lead acetate and to look for the reversibility of these changes after cessation of lead acetate and subsequent oral administration of honey. Experimental animal study. National Institute of Health Islamabad from January to June, 2009. Animals were obtained from the animal house of N.I.H and were divided into three groups A, B and C. Group A was subdivided into two groups A-l and A-ll. Group B was also subdivided into two sub groups; B-l and B-ll. Group C was not subdivided into subgroups. The animals in group A were used as control, while those of groups B and C were treated with lead acetate that was given intraperitonially in the dose of 4mg/kg body weight, 5 days a week for 6 weeks. The animals in group B-l were sacrificed at the end of six week to observe the toxic changes while animas in group B-ll were kept alive for another 6 weeks on normal diet. The animals in group C were given honey in dosage of 10ml/100ml water with normal diet for further Gweeks. These groups [B-l I and C] were then sacrificed after 12 weeks to observe the effects of honey on spermatogenesis. The histological comparison of testes of both groups of animals showed that after six weeks, the width of germinal epithelium and the number of spermatogenic cells had decreased in lead toxic groups as compared to the control rats [p<0.05] and in majority of the seminiferous tubules, the basement membrane was disrupted. The width of germinal epithelium, and the number of spermatogenic cells were improved after oral administration of honey. This study provides evidence that lead has toxic effects on testis which are partially reversible on oral intake of honey
ABSTRACT
To find out lead toxicity affects all levels of reproductive axis including both testosterone and Luteinizing Hormone production in adult male rats. An experimental study on animals The study was carried out at Islamic International Medical College, Rawalpindi from January 2010toNovember2010. Thirty adult male Sprawgue Dawley rats, purchased from NIH, Islamabad were divided into two groups A and B each containing 15 rats. Group A served as normal control receiving plain tap water. Group B received 0.3% lead acetate in drinking water. Five rats from each group were sacrificed at the end of 2nd, 4th and 6th weeks. Serum testosterone and LH levels were analyzed using ELISA technique. Results were analyzed using SPSS version 13. Serum testosterone level was significantly decreased in lead treated group as compared to control group whereas LH levels showed no significant change. Lead toxicity in male rats suppressed serum testosterone levels at all duration whereas LH levels at all durations manifested no significant change. This might be a result of direct testicular toxicity acting at testicular enzyme level alone or involving the hypothalamic-pituitary axis as well
ABSTRACT
The objective of this study was to observe changes in spermatogenesis in the testes of albino rats exposed to lead acetate. An experimental animals study. This study was carried out in the Department of Anatomy, Islamic International Medical College Rawalpindiand at National Institute of Health Islamabad from January to April, 2009. Male adult rats were exposed to lead acetate with intraperitoneal dose of 4mg/Kg body weight for 5 days a week for 6 weeks. The animals in group A were used as control. The animals of groups B were treated with lead acetate with specified dose for 6 weeks. After 6 weeks the animals of subgroup B were sacrificed. The results of these two groups were then compared, After six weeks, it was observed that the number of spermatogenic cells had decreased in the test groups as compared with control group [p<0.05]. Lead is toxic for cells of spermatogenic series, injurious to heaith and plays a significant role in reducing male fertility